Delivery System

The ability of Catalase-SKL to gain entry into the peroxisome and, once there, to metabolize hydrogen peroxide, is dependent on the enzyme first having accessed the cell interior. That is, catalase must be delivered inside the cell. To achieve this goal, EXT has adopted a protein “transduction” strategy, whereby Catalase-SKL is either mixed with a delivery molecule prior to the addition to cells, or a delivery motif is fused to the end of the catalase molecule (opposite of where the SKL signal is positioned). By either method, Catalase-SKL is transduced across the cell’s plasma membrane and enters the cytosol. Catalase-SKL is then poised to engage the peroxisomal protein import apparatus and enter the peroxisome. Experiments are currently underway to optimize Catalase-SKL delivery efficiencies for a wide range of cell and tissue types.